There are numerous methods for measuring 5-hydroxymethylcytosine (5-hmC) in DNA hydroxymethylation studies. The techniques can vary from complicated procedures such as Mass Spectrophotometry which require a significant amount of expertise to simpler ELISA-based methods suitable for a wide range of scientists from all levels of expertise and varying disciplines.
Comparing different samples and assessing 5-hmC can give researchers insight into numerous diseases and other epigenetic outcomes. Measuring global 5-hmC, for example, could give researchers more information about cancer development or the formation of neurological disorders. Reduction in total 5-hmC has been linked to several different cancer types and to the progression of major disorders. However, there is still much to be discovered about 5-hydroxymethylcytosine and its function is not yet entirely understood. Use these kits to unlock the mysteries behind this profound epigenetic mechanism.
DNA Hydroxymethylation Kits
MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)
An ideal product for researchers just beginning to explore epigenetic research, the MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit directly quantifies global 5-hydroxymethylcytosine in an ELISA-based format using a 96-well plate. See a table comparing the MethylFlash with alternative ELISA-based global DNA methylation assays.
EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit
For antibody-based capture of hydroxymethylated DNA fragments for microarray, PCR, or NGS applications. Carry out a successful hMeDIP procedure for the selective enrichment of DNA fragments containing 5-hydroxymethylcytosine (5-hmC) in a high throughput format using DNA isolated from any species.
5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9]
Mouse monoclonal antibody to 5-Hydroxymethylcytosine (5-hmC), clone HMC/4D9. This high quality antibody can be used in various applications, including immunofluorescence, immunohistochemistry, ELISA, Dot Blot, and hMeDIP.
Epigenetic Services for Genome-Wide DNA Hydroxymethylation Analysis
Epigentek’s DNA hydroxymethylation service platforms will specifically detect 5-hydroxymethylcytosine (5-hmC) at either a multi base resolution or single base resolution without cross-reactivity to methylated cytosine or unmethylated cytosine. Services offered include hMeDIP-Seq, True 5-hmC Sequencing Service (Hydroxymethyl-Seq), and Global 5-hmC Quantification.